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Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig T… | B-LABO France
Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit
Réf. : BL-RE-107507Réf. fabricant : 17717443 5 x 96 reactions
2 195,00 € HT
Le kit ELISA pour Ig totale anti-SARS-CoV-2 Spike Dynabeads™ est un essai immuno-enzymatique (ELISA) en sandwich en phase solide conçu pour détecter et quantifier le taux d’IgG, d’IgM et d’IgA humaines anti-SARS-CoV-2 dans les échantillons de sérum et de plasma.Le kit peut être utilisé pour les dosages qualitatifs et quantitatifs
Certifications/Conformité
ISO 9001
Contenu et stockage
Kit contains 5X of the following reagents: • Dynabeads SARS-CoV-2 Spike, 3 mL (1 mg/mL) • Human SARS-CoV-2 total Ig High Control antibody, 1 vial (lyophilized) • Human SARS-CoV-2 Total Ig Low Control antibody, 1 vial (lyophilized) • Human SARS-CoV-2 Total Ig Calibrator (medium control) antibody, 1 vial (lyophilized) • Wash buffer (20X concentrated), 50 mL • Assay buffer (20X concentrated), 50 mL • Substrate solution (tetramethylbenzidine), 15 mL • Stop solution, 15 mL • Human SARS-CoV-2 Total Ig HRP Conjugate, 0.12 mL
Store at 2–8°C.
Méthode de détection
Absorbance at 450 nm
Type de produit
SARS-CoV-2 ELISA Ig Total Kit
À utiliser avec (application)
Binding of human immunoglobulins (IgG, IgM, IgA) towards SARS-CoV-2 (Spike trimer)
Marque
Invitrogen
Caractéristiques principales
A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples
The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
Dynabeads SARS-CoV-2 Spike beads can be purchased separately (18100D) for other uses such as the optimization of unique conditions
Using Dynabeads and automation drastically improves reproducibility, limits hands on time, and reduces operating errors
The intra-assay CV value is typically <5% (range 2.8–3.2%), while the inter-assay CV is <15% (range 3.9–9.3%)
Kit can be used for both qualitative and quantitative assays
One of the fastest ELISAs for SARS-CoV-2 serology
For research use only, not for use in diagnostic procedures
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-107507
Réf. fabricant
17717443
Certifications/Conformité
ISO 9001
Contenu et stockage
Kit contains 5X of the following reagents:<br />• Dynabeads SARS-CoV-2 Spike, 3 mL (1 mg/mL)<br />• Human SARS-CoV-2 total Ig High Control antibody, 1 vial (lyophilized)<br />• Human SARS-CoV-2 Total Ig Low Control antibody, 1 vial (lyophilized)<br />• Human SARS-CoV-2 Total Ig Calibrator (medium control) antibody, 1 vial (lyophilized)<br />• Wash buffer (20X concentrated), 50 mL<br />• Assay buffer (20X concentrated), 50 mL<br />• Substrate solution (tetramethylbenzidine), 15 mL<br />• Stop solution, 15 mL<br />• Human SARS-CoV-2 Total Ig HRP Conjugate, 0.12 mL<br /><br>Store at 2–8°C.<br><br>
Méthode de détection
Absorbance at 450 nm
Type de produit
SARS-CoV-2 ELISA Ig Total Kit
À utiliser avec (application)
Binding of human immunoglobulins (IgG, IgM, IgA) towards SARS-CoV-2 (Spike trimer)
À utiliser avec (équipement)
KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
Quantité
5 x 96 reactions
Conditions d’expédition
Wet ice (2° to 8°C)
Diamètre (métrique) des microbilles
2.7 μm
Matériau
Monosized, superparamagnetic polystyrene magnetic beads
Compatibilité à haut débit
Yes, on KingFisher™ Flex or Apex instruments
Une question, une configuration particulière ? Contactez-nous pour un devis.
