For detection and measurement of human interleukin 10
Description
Dendritic Cells; Macrophages; Monocytes; T Cells, CD4+
À utiliser avec (application)
ELISA
Quantité
2 Plates
Marque
STEMCELL Technologies
Caractéristiques principales
The Human Interleukin 10 (IL-10) ELISA Kit is designed for the quantitative detection and measurement of human IL-10 in biological fluids such as serum, plasma, and cell culture supernatants
IL-10 is primarily produced by dendritic cells, macrophages, and CD4+ T cells
IL-10 is important for the function of regulatory T cells and it enhances B cell proliferation, immunoglobulin secretion, and MHC class II expression
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine
The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody
Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-108094
Réf. fabricant
17128021
À utiliser avec (application)
ELISA
Quantité
2 Plates
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