For detection and measurement of human immunoglobulin E
Description
B Cells; Hybridomas
Quantité
2 Plates
Marque
STEMCELL Technologies
Caractéristiques principales
The Human Immunoglobulin E (IgE) ELISA Kit is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants
IgE is found in the lungs, skin, and mucous membranes
IgE plays a role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI)
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin
The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody
Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-108096
Réf. fabricant
17158041
Quantité
2 Plates
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