Kit ELISA sandwich
The Human c-Met (Total) ELISA Kit is designed to detect and quantify the level of c-Met (Total) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant full length human c-Met (Total), independent of its phosphorylation state. Principle of the method A monoclonal capture antibody specific for c-Met (Total) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
• Une large gamme de kit ELISA chez l'humain, la souris, le singe, le boeuf et le porc pour différents types d'échantillons (tissus, lysat cellulaire, etc.)
• Test effectué en 3h maximum
• Pour toute demande, veuillez nous consdulter au 0388675323
Conditions d’expédition : Glace humide
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-108118 |
| Réf. fabricant | 10272643 |
| Numéro d’adhésion | P08581 |
| Plage de dosage | 0,78-50 ng/ml |
| Sensibilité du dosage | <0,4 ng |
| Concentration | 0,78 à 50 ng/ml |
| Conjugué | HRP |
| Type de produit | Kit ELISA |
| Type d’échantillon | Lysats cellulaires |
| Type de substrat | Tyrosine kinases de récepteur |
| Espèces cibles | Humaine |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 4233 |
| Symbole de gène(s) | AUTS9,DFNB97,HGFR,RCCP2,c-Met |
| Temps d’incubation | 4 h |
| Coefficient de variation inter-dosage | 10,8% |
| Coefficient de variation intra-dosage | 8,6% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Tampon de dilution standard, Détection d'anticorps, Anti-lapin HRP, Diluant HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| Marqueur génétique | c-Met |
| Quantité | 96 tests |
| État réglementaire | RUO |
| Volume d’échantillon | Lysat cellulaire ,10 μl |
| Conditions de stockage | 2°C à 8°C |
| Cible | Humaine |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 4 h |
