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Thermo Scientific™ Pierce™ Kit ELISA IL-12 (total) de souriss

Name: Thermo Scientific™ Pierce™ Kit ELISA IL-12 (total) de souriss
Brand: Thermo Scientific Pierce
SKU: 10371204
Price: 560.70 EUR
Availability: InStock

Réf. : BL-RE-108138Réf. fabricant : 10371204 2 Plate Kit

560,70 € HT

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Plage de dosage: 47 to 3000pg/mL
Sensibilité du dosage: <12pg/mL
Description: Mouse IL-12 Total (p70, p40) ELISA Kit
Format du kit d’immunoessai: Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque: Thermo Scientific Pierce

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Caractéristiques principales

Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 47 to 3000pg/mL
Sensitivity: < 12pg/mL
Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Caractéristiques techniques

Marque	Thermo Scientific Pierce
Réf. B-LABO	BL-RE-108138
Réf. fabricant	10371204
Plage de dosage	47 to 3000pg/mL
Sensibilité du dosage	<12pg/mL
Format du kit d’immunoessai	Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon	Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Spécificité	No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
Suffisant pour	2 x 96 microplate wells
Contenus des kits	TMB Substrate Solution, 2 x 12mL, Adhesive Plate Covers, 12 ea, Anti-Mouse IL-12 Total Precoated 96-well Strip Plate, 2 ea, Streptavidin-HRP Concentrate, 2 x 75µL, Stop Solution, 2 x 13mL, Standard Diluent, 2 x 20mL, Lyophilized Recombinant Mouse IL-12 Standard, 4 vials, HRP Dilution Buffer, 2 x 13mL, 30X Wash Buffer, 2 x 50mL, Biotinylated Antibody Reagent, 2 x 12mL
Mesure	Absorbance at 450nm minus absorbance at 550nm
Précision	Dependent upon sample type (generally, intra- and inter-assay CV <10%)
Quantité	2 Plate Kit
Volume d’échantillon	50 μL
Cible	Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Durée totale du dosage	3 hr.

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Thermo Scientific™ Pierce™ Kit ELISA IL-12 (total) de souriss

Caractéristiques principales

Caractéristiques techniques

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