The Thermo Scientific Human IL-2R ELISA Kit is used to measure s-IL-2R (human soluble interleukin-2 receptor) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatants. Each kit contains two anti-Human sIL-2R capture antibody precoated 96-well Strip Plates and sufficient detection antibody, conjugate, buffers, diluent, TMB and stop buffers, as well as lyophilized sIL-2R standard to analyze several samples.
Points forts
Features of the Thermo Scientific Human IL-2R ELISA Kit
Target: natural and recombinant human soluble interleukin-2 receptor (sIL-2R); also called CD25
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Caractéristiques techniques
| Marque | Thermo Scientific |
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| Réf. B-LABO | BL-RE-108140 |
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| Réf. fabricant | 15388194 |
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| Plage de dosage | 0 to 6500U/mL |
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| Sensibilité du dosage | <24U/mL |
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| Méthode de détection | Colorimetric |
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| Format du kit d’immunoessai | Strip Plate |
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| Type de produit | IL-2R ELISA Kit |
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| Famille de protéines | Cytokines and receptors |
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| Type d’échantillon | Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum |
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| Spécificité | No significant cross-reaction with human IL-1α±, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα±, TNFβ, or mouse IL-2 |
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| Espèces cibles | Human |
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| À utiliser avec (application) | Protein assays and analysis |
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| Contenus des kits | Detector antibody, Diluents, HRP Conjugate, Pre-coated plate(s), Standard or calibrator, Stop solution, Substrate, Wash buffer |
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| Mesure | Absorbance at 450nm minus absorbance at 550nm |
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| Précision | Intra-assay CV ≤10%; inter-assay CV ≤10% |
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| Quantité | 192 Assays |
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| État réglementaire | For research use only (RUO) |
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| Disciplines de recherche | Immunology |
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| Volume d’échantillon | 50 μL |
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| Conditions de stockage | 2°C to 8°C |
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| Cible | CD25 |
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| Durée totale du dosage | 2.5 hr. |
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