Kit ELISA instantané
The Human RANTES(Hu regulated upon activation, normal T-cell expressed and secreted)) ELISA quantitates Hu RANTES in human serum, plasma, cell culture supernatants, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu RANTES. Principle of the method The Human RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Reconnaît l’IP-10 naturelle et recombinante.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-108151 |
| Réf. fabricant | 15561887 |
| Numéro d’adhésion | P13501 |
| Plage de dosage | 31,3-2,000 pg/ml |
| Sensibilité du dosage | 4,2 pg/ml |
| Conjugué | Biotine |
| Type de produit | Kit ELISA |
| Type d’échantillon | Liquide amniotique, Sérum, Surnageant, Supernatant |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 6352 |
| Symbole de gène(s) | D17S136E,RANTES,SCYA5,SIS-delta,SISd,TCP228,eoCP |
| Coefficient de variation inter-dosage | 9.9% |
| Coefficient de variation intra-dosage | 6.9% |
| Contenus des kits | Tampon de lyse 10x, Couvercles de plaques adhésives, Anticorps de détection biotinylé, Anticorps de détection biotinylé, lyophilisé, Solution d’arrêt, Diluant d’échantillon, Diluant d’échantillon, Concentrat de tampon de lavage, Concentrat de tampon de lavage, Solution de substrat |
| Marqueur ou colorant | HRP |
| Quantité | 128 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | Liquide amniotique, 10 μl ; plasma, 10 μl ; sérum, 10 μl ; surnageant, 10 μl |
| Conditions de stockage | -20°C |
| Synonyme | Cytokine A5 inductible de petite taille, SIS-delta, CCL5 |
| Cible | Humaine |
| Heure du test | 20 min |
| Durée totale du dosage | 3 h 10 min |
