The ProcartaPlex Human IGF-1R Simplex Kit measures the level of total IGF-1R in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
Type de produit
Simplex Kit
Quantité
96 Tests
Espèces
Human
Combinability
Combinable
Type de microbilles
Magnetic
Marque
Invitrogen
For preparation of cell lysates, we recommend use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-999999-901) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) (Cat. No. 78440). These buffers are not included with the panel. Depending on your cell system, other buffers may be suitable as well.
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About ProcartaPlex Assays for the Luminex Platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum or 50 μL of cell culture supernatant and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatant, cell lysate, and other bodily fluids.
More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation
