Cell or tissue lysates are a useful control for the western blotting application
À utiliser avec (application)
Western Blot
Tissue
Epidermoid Carcinoma
Quantité
500 μg
Tampon de stockage
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Espèces hôtes
Human
Marque
BD
A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. A431 cells (Human epithelial carcinoma; ATCC CRL-1555) were grown to 80-90% confluency, serum starved overnight, then treated with 100ng/mL EGF for 5 minutes at 37°C.
