BromoTag™ ABG3 is a selective Bump and Hole TAG Degrader (DC50 210 - 290 nM, protein degradation t½ = 113 min, Dmax = 92%). BromoTag AGB3 comprises a VHL E3 ligase ligand joined by a linker to a (+)-JQ1 (Cat. No. 4499) derivative (thebump bearer) that selectively binds the Brd4BD2 L387A variant (thehole). In cell lines expressing a fusion of the target protein with the tag, Brd4BD2 L387A, BromoTag AGB3 drives formation of a ternary complex between VHL and theBromoTagged protein, leading to ubiquitination of the protein and its subsequent proteasomal degradation. BromoTag™ is a registered trademark of the University of Dundee and is used under license. Brd4BD2 L387A can be expressed as a fusion with a target protein of interest using genome engineering techniques via CRISPR-mediated locus-specific knock-in. See protocol for more information.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-125325 |
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| Réf. fabricant | 18045332 |
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| Nom chimique ou matériau | (2S,4R)-1-((2S,17R)-2-(tert-Butyl)-17-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-4,16-dioxo-6,9,12-trioxa-3,15-diazanonadecanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide |
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| CAS | 2776191-07-0 |
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| Quantité | 5 mg |
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| Cible | TAG Degradation Platforms |
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| Formule moléculaire | C<sub>51</sub>H<sub>64</sub>ClN<sub>9</sub>O<sub>8</sub>S<sub>2</sub> |
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| Pureté | 98% |
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