preQ1 with alkyne handle for click chemistry
preQ1-alkyne is a modified analog of the natural substrate prequeosine1 (preQ1). It contains alkyne handle that enables users to attach additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. It can be used to generate photoactivatable probes that can control CRISPR gene editing via the RNA-TAG method.
Other RNA-TAG toolbox compounds include preQ1-biotin ( Cat. No. 7804 )
Plasmids for expression of the E.coli TGT enzyme ( #138201 ) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end ( #138209 ) are available from Addgene.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-125603 |
| Réf. fabricant | 18796016 |
| Nom chimique ou matériau | N-[6-[[(2-Amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]amino]hexyl]-3-(2-propyn-1-yloxy)propanamide |
| CAS | 2771096-76-3 |
| Quantité | 10 mg |
| Cible | CRISPR Reagents |
| Formule moléculaire | C<sub>19</sub>H<sub>28</sub>N<sub>6</sub>O<sub>3</sub> |
