Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
2A3 is an electrophilic chemical probe that acylates the 2'-OH in the RNA backbone. 2A3 can be used for RNA SHAPE-MaP experiments in cell lines or live bacteria. It labels single-stranded or flexible regions more than folded regions (SHAPE methodology) and enables analysis of RNA secondary structures at single nucleotide resolution. For intracellular experiments, 2A3 has higher signal-to-noise ratio and prediction accuracy than NAI (Cat. No. 7004). 2A3 is cell permeable, and can probe both gram-positive and gram-negative bacteria.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-125891 |
| Réf. fabricant | 18728786 |
| Nom chimique ou matériau | (2-Aminopyridin-3-yl)(1 H-imidazol-1-yl)methanone |
| Quantité | 10 mg |
| Cible | DNA, RNA and Protein Synthesis Compounds |
| Formule moléculaire | C<sub>9</sub>H<sub>8</sub>N<sub>4</sub>O |
