RNA conformation probe; reagent for COMRADES RNA platform
Psoralen TEG Azide is used to probe RNA structure and conformation in live cells, using the cross-linking of matched RNAs and deep sequencing or COMRADES method. Psoralen crosslinks the RNA and the azide enables subsequent click reaction with biotin. The COMRADES method has been used to determine the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. It has also been used to map RNA-RNA interactions along the SARS-CoV-2 genome and subgenomes inside cells. Psoralen TEG Azide is cell permeable. For detailed methods please see references section below.
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-125892 |
| Réf. fabricant | 18739186 |
| Nom chimique ou matériau | 3-(13-Azido-5,8,11-trioxa-2-azatridec-1-yl)-2,5,9-trimethyl-7 H-furo[3,2-g][1 ]benzopyran-7-one |
| CAS | 1352815-11-2 |
| Quantité | 5 mg |
| Cible | DNA, RNA and Protein Synthesis Compounds |
| Formule moléculaire | C<sub>23</sub>H<sub>30</sub>N<sub>4</sub>O<sub>6</sub> |
| Pureté | 0.98 |
