Janelia Fluor® 525, SE is a yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Can be multiplexed for two color imaging with Janelia Fluor™ 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 525 nm; emission maximum = 549 nm; quantum yield = 0.91; Extinction coefficient = 122,000 M-1cm-1; Correction factor A280 = 0.185.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-126267 |
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| Réf. fabricant | 18794089 |
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| Nom chimique ou matériau | 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt |
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| Quantité | 2 mg |
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| Cible | Amine-Reactive Janelia Fluor™ Dyes |
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| Formule moléculaire | C<sub>31</sub>H<sub>21</sub>F<sub>4</sub>N<sub>3</sub>O<sub>7</sub> |
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