Purify phosphorylated peptides to process protein digests or cation-exchange fractions for mass spectrometry using spin columns of iron-chelate agarose resin.
Processing 30 samples (200µL each) containing up to 150µg of phosphopeptides each
Quantité
30 Column Kit
Marque
Thermo Scientific
Thermo Scientific™ Pierce Fe-NTA Phosphopeptide Enrichment Kit enables fast and efficient enrichment of phosphorylated peptides to process protein digests or strong cation-exchange peptide fractions for analysis by mass spectrometry.
The Pierce Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. These columns enrich a higher percentage of phosphopeptides than other resins and with an overall higher number of total and unique phosphopeptides. The complete kit is easy to use and requires less than one hour to process protein digests or strong cation-exchange peptide fractions for analysis by mass spectrometry (MS).
Points forts
Highlights
Convenient — spin-column format enables parallel processing of multiple samples
High capacity — each column enriches up to 150μg of phosphopeptides
Excellent recovery — our kit enriches more total and unique phosphopeptides than other commercially available resins
High coverage — recover peptides with single and multiple phosphorylation sites
Product Details
To achieve robust MS results, enrichment of phosphopeptide samples is essential because of low abundance and poor ionization relative to non-phosphorylated peptides. Multiple phosphorylated amino acids within a peptide contribute to the complexity of phosphopeptide analysis. The Pierce Fe-NTA Spin Columns effectively enriches singly phosphorylated peptides as well as peptides with three or more phosphorylated sites, reducing sample complexity and enabling effective identification and characterization of phosphorylated peptides by MS. The Pierce Fe-NTA Phosphopeptide Enrichment Kit contains detailed instructions and all necessary components to load, wash and elute phosphopeptides within an hour. This kit is compatible with samples digested in solution or after in-gel digestion. Using the Pierce Graphite Spin Columns (Part No. 88302) after enrichment will improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before MS analysis.
