CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix.
Capacité de liaison
400 nmol/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store at 2°C to 8°C.
Conditions d’expédition
Room Temperature
Marque
Thermo Scientific
Features of C-tagXL Affinity Matrix include:
CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl2 pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A.
Caractéristiques principales
Mild elution, making it suitable for pH-sensitive proteins
Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
Excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127433
Réf. fabricant
18406798
Capacité de liaison
400 nmol/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store at 2°C to 8°C.
Conditions d’expédition
Room Temperature
Granulométrie
65 ± 10 μm
Forme
Liquid Suspension
Gamme de produits
CaptureSelect™
Quantité
5 mL
Type
Affinity Matrix
Type de ligand
C-tagXL affinity ligand
Phase stationnaire
Anti-EPEA tag
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