CaptureSelect FcXP Affinity Matrix can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in one step.
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect FcXP affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl2, 40% (v/v) propylene glycol, pH 7
Caractéristiques principales
Mild elution, making it suitable for Fc fusion proteins
Human-specific, no binding to bovine antibodies
Excellent scalability
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127464
Réf. fabricant
18426448
Capacité de liaison
40 to 50 g IgG/L resin
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide activated
Granulométrie
65 ± 10 μm
Forme
Resin Slurry
Gamme de produits
CaptureSelect™
Quantité
50 mL
Type
Affinity Matrix
Type de ligand
FcXP affinity ligand
Une question, une configuration particulière ? Contactez-nous pour un devis.
