CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG.
Capacité de liaison
25 to 35 g IgG/L resin
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose based, aldehyde activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Granulométrie
65 ± 10 μm
Marque
Thermo Scientific
Features of this affinity matrix include:
CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main Characteristics:
Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect™ FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Caractéristiques principales
Mild elution, making it suitable for Fc fusion proteins
Human specific, no binding to bovine antibodies, for example
Excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127567
Réf. fabricant
18446688
Capacité de liaison
25 to 35 g IgG/L resin
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose based, aldehyde activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Granulométrie
65 ± 10 μm
Forme
Liquid Suspension
Gamme de produits
CaptureSelect™
Quantité
5 mL
Type
Affinity Matrix
Type de ligand
FcXL affinity ligand
Phase stationnaire
Anti-IgG-Fc
Une question, une configuration particulière ? Contactez-nous pour un devis.
