Like C-tag Affinity Matrix, C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.
The affinity matrix is offered in a pre-packed format as a set of 5 x 1 mL columns or as one 5 mL column, with connectors that are compatible with standard chromatography equipment.
Caractéristiques techniques
| Marque | Thermo Scientific |
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| Réf. B-LABO | BL-RE-127578 |
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| Réf. fabricant | 18416528 |
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| Contenu et stockage | Store in refrigerator (2°C to 8°C). |
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| Conditions d’expédition | Room Temperature |
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| Forme | Liquid Suspension |
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| Gamme de produits | CaptureSelect™ |
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| Quantité | 5 x 1 mL Columns |
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| Type | Pre-packed Column |
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| Phase stationnaire | Anti-C-tagXL |
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