CaptureSelect HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes.
Concentration
0.5 mg/mL
Formule
Liquid, in PBS (7.4), 50% glycerol
Contenu et stockage
Store in freezer (-5°C to -30°C).
Forme
Liquid
Gamme de produits
CaptureSelect™
Marque
Thermo Scientific
CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (cat. no. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.
For assay development to measure AAV9 total capsids, we recommend CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) and CaptureSelect HRP Anti-AAV9 Conjugate (Cat. No. 7303332100).
Points forts
Product details
Format type: HRP, liquid
Buffer: PBS, 50% (v/v) glycerol
Storage: in freezer (-5 to -30°C)
Concentration: 0.5 mg/mL
Volume: 0.1 mL
Recommended working dilution for sandwich ELISA: 1: 5000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127614
Réf. fabricant
18436658
Concentration
0.5 mg/mL
Formule
Liquid, in PBS (7.4), 50% glycerol
Contenu et stockage
Store in freezer (-5°C to -30°C).
Forme
Liquid
Gamme de produits
CaptureSelect™
Quantité
100 μL
À utiliser avec (application)
Quantitation of multiple AAV capsids
Type
Conjugate
Une question, une configuration particulière ? Contactez-nous pour un devis.
