Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.
Capacité de liaison
4.0 mg/mL
Capacité (métrique)
1.7 mL
Débit
Up to 5000 cm/hr.
Matrice
Polystyrenedivinylbenzene
Porosité
0.16 micron
Marque
Thermo Scientific
POROS™CaptureSelect™ IgM HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three minute quantitation of IgM antibodies.
Features:
Points forts
High Speed with High Specificity
The combination of CaptureSelect™ IgM affinity ligands when combined with POROS™ 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of IgM.
POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.
Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
