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Thermo Scientific™ CaptureSelect™ KappaXP MiniChrom Column

Réf. : BL-RE-127713Réf. fabricant : 18446668 1 Column
1 440,00 € HT

The CaptureSelect KappaXP MiniChrom column is designed for bench-scale resin screening, process development, and sample preparation.

Volume du lit
5 mL
Débit
75 to 150 cm/hr. (Up to 2 bar)
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Diamètre (métrique)
0.8 cm
Longueur (métrique)
10 cm
Marque
Thermo Scientific

The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.

CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include:

Points forts

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect KappaXP affinity ligand
  • Ligand coupling method: epoxide coupling
  • Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
  • IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height

Caractéristiques principales

  • Volume of 5 mL (0.8 x 10 cm)
  • Direct connection to standard chromatography systems
  • Packed with CaptureSelect KappaXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates
  • Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
  • Mild elution for Fab fragments and antibodies
  • Human-specific, no binding to bovine antibodies
  • Excellent scalability
  • Excellent pH stability up to 100 mM NaOH (limited exposure)
  • Non-animal derived

Caractéristiques techniques

MarqueThermo Scientific
Réf. B-LABOBL-RE-127713
Réf. fabricant18446668
Volume du lit5 mL
Débit75 to 150 cm/hr. (Up to 2 bar)
Contenu et stockageStore in refrigerator (2°C to 8°C).
Diamètre (métrique)0.8 cm
Longueur (métrique)10 cm
Conditions d’expéditionWet Ice
Granulométrie65 ± 10 μm
Gamme de produitsCaptureSelect™
Quantité1 Column
TypePre-packed Column
Type de ligandKappaXP affinity ligand
Phase stationnaireKappaXP Affinity Matrix with KappaXP Ligand
FormatChromatography Cartridge
Type de colonneMiniChrom
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