The CaptureSelect KappaXP MiniChrom column is designed for bench-scale resin screening, process development, and sample preparation.
Volume du lit
5 mL
Débit
75 to 150 cm/hr. (Up to 2 bar)
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Diamètre (métrique)
0.8 cm
Longueur (métrique)
10 cm
Marque
Thermo Scientific
The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.
CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.
Features of this affinity matrix include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height
Caractéristiques principales
Volume of 5 mL (0.8 x 10 cm)
Direct connection to standard chromatography systems
Packed with CaptureSelect KappaXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates
Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
Mild elution for Fab fragments and antibodies
Human-specific, no binding to bovine antibodies
Excellent scalability
Excellent pH stability up to 100 mM NaOH (limited exposure)
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127713
Réf. fabricant
18446668
Volume du lit
5 mL
Débit
75 to 150 cm/hr. (Up to 2 bar)
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Diamètre (métrique)
0.8 cm
Longueur (métrique)
10 cm
Conditions d’expédition
Wet Ice
Granulométrie
65 ± 10 μm
Gamme de produits
CaptureSelect™
Quantité
1 Column
Type
Pre-packed Column
Type de ligand
KappaXP affinity ligand
Phase stationnaire
KappaXP Affinity Matrix with KappaXP Ligand
Format
Chromatography Cartridge
Type de colonne
MiniChrom
Une question, une configuration particulière ? Contactez-nous pour un devis.