Features of the CaptureSelect Factor X Affinity Matrix include:
Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect Factor X affinity ligand
Ligand coupling method: epoxide
Binding capacity: >10 mg/mL resin
Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
Caractéristiques principales
Highly selective for human Factor X with no affinity for other human plasma proteins
Binds both Factor X and Factor Xa in the presence of calcium
Enables efficient purification of Factor X with high yields and retained activity
Mild elution at neutral pH using EDTA
Offers excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127730
Réf. fabricant
18456608
Capacité de liaison
>10 mg/mL
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Conditions d’expédition
Wet Ice
Granulométrie
65 ± 10 μm
Forme
Agarose Bead
Gamme de produits
CaptureSelect™
Quantité
50 mL
Cible
Protein
Type
Affinity Matrix
Type de ligand
Factor X affinity ligand
Phase stationnaire
Factor X
Type de colonne
Agarose Resin
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