CaptureSelect C-tagXL MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation.
Capacité de liaison
400 nmol/mL
Capacité (métrique)
5 mL
Débit
75 to 150 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Marque
Thermo Scientific
The 1-mL columns can be used for quick screening of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.
CaptureSelect C-tagXL Affinity Matrix has a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine). CaptureSelect C-tagXL Affinity Matrix purifies the C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high-activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.
Features of this affinity matrix include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4; 100 mM glycine buffer, pH 2–3. Neutral (pH 7.0–7.4): 20 mM Tris, 2.0 M MgCl2; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris, 2 mM 'S-E-P-E-A' peptide
Caractéristiques principales
Dimensions of 1 mL (0.8 cm x 2 cm) and 5 mL (0.8 cm x 10 cm)
Direct connection to standard chromatography systems
Packed with CaptureSelect C-tagXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates
Mild elution, making it suitable for pH-sensitive proteins
Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
Excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127780
Réf. fabricant
18466528
Capacité de liaison
400 nmol/mL
Capacité (métrique)
5 mL
Débit
75 to 150 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Diamètre (métrique)
0.8 cm
Longueur (métrique)
10 cm
Conditions d’expédition
Wet Ice
Granulométrie
65 ± 10 μm
Gamme de produits
CaptureSelect™
Quantité
1 Ea.
Type
Column
Type de ligand
C-tagXL affinity ligand
Phase stationnaire
C-tagXL
Type de colonne
MiniChrom
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