POROS CaptureSelect BacuClear Affinity Matrix is specifically designed for the removal of baculovirus contaminants when using baculovirus expression systems (BEVS) for the production of recombinant biomolecules.
Capacité de liaison
>1 x 1010 VG/mL of resin (estimated)
Formule
50% slurry suspension in 20% ethanol
Matrice
Cross-linked poly(styrene-divinylbenzene)
Contenu et stockage
Store in refrigerator (2°C to 8°C). Protect from daylight
Pression de fonctionnement max.
100 bar
Marque
Thermo Scientific
To remove baculovirus impurities, the affinity matrix is operated in flow-through mode. As described by Moleirinho et. al. (1), successful removal of baculovirus particles from influenza VLPs, HCV VLPs, and AAVs was achieved with the affinity ligand identified (BV-2) coupled to the POROS matrix. A dynamic binding capacity for baculovirus particles of 1.6 x 1010 VG/mL affinity matrix was demonstrated with baculovirus removals observed above 70% and VLP recovery yields above 60%.
Features of this affinity matrix include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
1. M.G. Moleirinho, R.P. Fernandes, S.B. Carvalho, S. Bezemer, F. Detmers, P Hermans, R.J.S. Silva, P.M. Alves, M.J.T. Carrondo, C. Peixoto. Baculovirus affinity removal in viral-based bioprocesses. Sep. Purif. Technol. 241 (2020)
Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 μm
Ligand: CaptureSelect BacuClear affinity ligand
Ligand coupling method: epoxide
Binding capacity: estimated >1 x 1010 VG/mL of resin
Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2
Caractéristiques principales
One-step removal of baculovirus virus particles from virus and VLP containing samples, in flow-through mode
Enables a highly selective polishing step for baculovirus removal
High specificity, capacity, and retained activity
Excellent scalability
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127782
Réf. fabricant
18476598
Capacité de liaison
>1 x 10<sup>10</sup> VG/mL of resin (estimated)
Formule
50% slurry suspension in 20% ethanol
Matrice
Cross-linked poly(styrene-divinylbenzene)
Contenu et stockage
Store in refrigerator (2°C to 8°C). Protect from daylight
Pression de fonctionnement max.
100 bar
Conditions d’expédition
Ships at 2°C to 8°C
Granulométrie
50 μm
Forme
Resin Slurry
Gamme de produits
CaptureSelect™
Quantité
5 mL
Type
Affinity Matrix
Type de ligand
BacuClear affinity ligand
Phase stationnaire
POROS bead
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