Réf. : BL-RE-127784Réf. fabricant : 18496598 50 mL
Prix sur devis
Tarif personnalisé selon la configuration et la quantité.
POROS CaptureSelect BacuClear Affinity Matrix is specifically designed for the removal of baculovirus contaminants when using baculovirus expression systems (BEVS) for the production of recombinant biomolecules.
Capacité de liaison
>1 x 1010 VG/mL of resin (estimated)
Formule
50% slurry suspension in 20% ethanol
Matrice
Cross-linked poly(styrene-divinylbenzene)
Contenu et stockage
Store in refrigerator (2°C to 8°C). Protect from daylight
To remove baculovirus impurities, the affinity matrix is operated in flow-through mode. As described by Moleirinho et. al. (1), successful removal of baculovirus particles from influenza VLPs, HCV VLPs, and AAVs was achieved with the affinity ligand identified (BV-2) coupled to the POROS matrix. A dynamic binding capacity for baculovirus particles of 1.6 x 1010 VG/mL affinity matrix was demonstrated with baculovirus removals observed above 70% and VLP recovery yields above 60%.
Features of this affinity matrix include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
1. M.G. Moleirinho, R.P. Fernandes, S.B. Carvalho, S. Bezemer, F. Detmers, P Hermans, R.J.S. Silva, P.M. Alves, M.J.T. Carrondo, C. Peixoto. Baculovirus affinity removal in viral-based bioprocesses. Sep. Purif. Technol. 241 (2020)
Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 μm
Ligand: CaptureSelect BacuClear affinity ligand
Ligand coupling method: epoxide
Binding capacity: estimated >1 x 1010 VG/mL of resin
Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2
Caractéristiques principales
One-step removal of baculovirus virus particles from virus and VLP containing samples, in flow-through mode
Enables a highly selective polishing step for baculovirus removal
High specificity, capacity, and retained activity
Excellent scalability
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127784
Réf. fabricant
18496598
Capacité de liaison
>1 x 10<sup>10</sup> VG/mL of resin (estimated)
Formule
50% slurry suspension in 20% ethanol
Matrice
Cross-linked poly(styrene-divinylbenzene)
Contenu et stockage
Store in refrigerator (2°C to 8°C). Protect from daylight
Pression de fonctionnement max.
100 bar
Conditions d’expédition
Ships at 2°C to 8°C
Granulométrie
50 μm
Forme
Resin Slurry
Gamme de produits
CaptureSelect™
Quantité
50 mL
Type
Affinity Matrix
Type de ligand
BacuClear affinity ligand
Phase stationnaire
POROS bead
Une question, une configuration particulière ? Contactez-nous pour un devis.
