CaptureSelect IgG4 (Hu) Affinity Matrix has been specifically designed for the purification and isolation of human IgG4 from complex sources such as plasma, serum, or cell culture supernatants.
Capacité de liaison
>15 g IgG4/L resin
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide activated
Granulométrie
65 ± 10 μm
Forme
Resin Slurry
Marque
Thermo Scientific
Features of this affinity matrix include:
Points forts
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect IgG4 affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 g IgG4/L resin depending on flow rate, column height, and contact time
Elution conditions: 0.1 M glycine, pH 3.0
Caractéristiques principales
Highly selective for human IgG4
No cross-reactivity with other antibody subclasses (IgA, IgD IgE, IgM) or antibody subtypes (IgG1-3)
No cross reactivity with other species, allowing use of complex growth media containing fetal calf or horse serum
Excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127822
Réf. fabricant
18496738
Capacité de liaison
>15 g IgG4/L resin
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide activated
Granulométrie
65 ± 10 μm
Forme
Resin Slurry
Gamme de produits
CaptureSelect™
Quantité
5 mL
Type
Affinity Matrix
Type de ligand
IgG4 affinity ligand
Une question, une configuration particulière ? Contactez-nous pour un devis.
