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Thermo Scientific™ CaptureSelect™ CH1-XL RoboColumn

Réf. : BL-RE-127846Réf. fabricant : 18446578
514,00 € HT

CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

Capacité de liaison
>15 mg/mL human Fab fragment
Capacité (métrique)
200 μL
Débit
16 to 150 cm/hr.
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store in refrigerator (2°C to 8°C)
Marque
Thermo Scientific

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:

Points forts

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

RoboColumn is a trademark of Repligen.

  • Column size: 200 μL and 600 μL, 1 strip, 8 columns (8-row)
  • Column dimension: 200 uL: 5 mm D x 10 mm L
  • Column dimension: 600 uL: 5 mm D x 30 mm L
  • Matrix: agarose based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect CH1-XL affinity ligand

Caractéristiques principales

  • Platform approach for purification of high quality and correctly assembled human Fab fragments
  • No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • Caustic cleaning with up to 50 mM NaOH
  • Excellent scalability
  • No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
  • Non-animal-derived

Caractéristiques techniques

MarqueThermo Scientific
Réf. B-LABOBL-RE-127846
Réf. fabricant18446578
Capacité de liaison>15 mg/mL human Fab fragment
Capacité (métrique)200 μL
Débit16 to 150 cm/hr.
MatriceAgarose-based, epoxide-activated
Contenu et stockageStore in refrigerator (2°C to 8°C)
Diamètre (métrique)5 mm
Pression de fonctionnement max.2 bar
Longueur (métrique)10 mm
Conditions d’expéditionRoom temperature, or on wet ice
Granulométrie65 ± 10 μm
Gamme de produitsCaptureSelect™
À utiliser avec (application)Chromatography
TypeColumn
Type de ligandCH1-XL affinity ligand
Phase stationnaireCH1-XL affinity ligand on agarose bead
Format8-well Strips
Type de colonneRoboColumn
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