POROS Oligo (dT)25 Affinity Resin was developed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. Unlike alternate approaches, the resin selectively captures the mRNA via the polyadenylated (polyA) tail using simple salt and water purification steps.
Features of POROS Oligo (dT)25 Affinity Resin include:
POROS Oligo (dT)25 Affinity Resin allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.
Pack sizes of 1 L and above come with a regulatory support package. Worldwide technical support is also available.
Points forts
Main characteristics
Oligo (dT) ligands are manufactured using a synthetic manufacturing process that are free of animal components.
Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
Average particle size: 50 μm
Ligand: poly(dT) 25mer with proprietary linker
Binding capacity: >2 mg mRNA per mL of resin
Binding conditions: 0.5–1 M salt, common buffers, neutral pH
Elution conditions: Low salt, common buffers, neutral pH
Caractéristiques principales
Easy mRNA purification from crude transcription mix to effectively remove plasmid DNA and other IVT components
Simplified workflow helps to maximize efficiency, thereby reducing complexity of subsequent polish steps
Excellent scalability
Non-animal derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-127851
Réf. fabricant
18416448
Capacité de liaison
>2 mg mRNA per mL of resin
Matrice
Cross-linked poly(styrene-divinylbenzene) POROS beads
Contenu et stockage
Store at 2°C to 8°C.
Conditions d’expédition
Wet Ice
Granulométrie
50 μm
Forme
Liquid Suspension
Gamme de produits
POROS™
Quantité
250 mL
Cible
mRNA
Type
Bulk Resin
Phase stationnaire
Poly(dT) 25mer with proprietary linker
Une question, une configuration particulière ? Contactez-nous pour un devis.
