EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Type de cellule
B Cells
À utiliser avec (application)
Cell Isolation
Quantité
1 x 109 cells
Marque
STEMCELL Technologies
The EasySep™ Mouse Pan-B Cell Isolation Kit is designed to isolate all B cells, including conventional B-2 B cells, B-1 B cells and plasma cells from single-cell suspensions of splenocytes or other tissues by negative selection
Unwanted cells are targeted for removal with biotinylated antibodies directed against non pan-B cells (CD4, CD8, CD11c, CD49b, CD90.2, Ly-6C/G (Gr-1), TER119) and streptavidin-coated magnetic particles (RapidSpheres™ )
Labeled cells are separated using an EasySep™ magnet without the use of columns
Desired cells are poured off into a new tube
This kit is compatible for use with cells from disease models where the malignant cells (B-CLL) express CD43 or CD11b
For isolation of conventional B cells only, we recommend using the EasySep™ Mouse B Cell Isolation Kit (Catalog #19854)
