Thermo Scientific™ Pierce Titanium Dioxide Magnetic Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads.
The Magnetic Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads. The titanium dioxide (TiO
2
) ligand on these magnetic particles selectively binds peptides containing phosphorylated serine (Ser), tyrosine (Tyr) or threonine (Thr), enabling selective phosphopeptide enrichment from protease-digested samples. The isolated phosphopeptides are compatible for analysis downstream by mass spectrometry. The binding, wash and elution buffers supplied in the kits ensure that maximum specificity and yield.
Points forts
Highlights
Complete MS-compatible kits – include ready-to-use binding, wash and elution buffers that are optimized for phosphopeptide enrichment and downstream analysis by MALDI and ESI mass spectrometry
Optimized for HTS – procedure validated for processing 1 to 96 samples at a time; complete entire assay in about 15 minutes using a KingFisher Flex Instrument
Stable affinity ligand – titanium dioxide is specially coated as a film on the magnetic particles
Selective – affinity system is selective for phosphorylated Ser, Tyr and Thr; exhibits minimal non-specific binding to acidic residues
Sensitive – affinity provides more than 1000 times greater sensitivity than traditional IMAC technologies; enables enrichment and MS-measurement of less than 100 fmol of phosphoprotein
Includes
Pierce TiO2 Magnetic Beads; Binding, wash and elution buffers; 96-well PCR plates for automated processing
Requires
Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device
