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Thermo Scientific™ Pierce™ SILAC Protein Qua… | B-LABO France
Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits
Réf. : BL-RE-129298Réf. fabricant : 11688833 1 Use Kit
652,00 € HT
Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.
Description
SILAC Protein Quantitation Kit - DMEM:F12
Formule
Multi-component kit with heavy and light L-Lysine and DMEM:F12 Media
Contenus des kits
L-Lysine-2HCl, 50mg, Dialyzed FBS, 2 x 50mL, DMEM:F12 Media for SILAC, 2 x 500mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6, 50mg
Suffisant pour
One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
Quantité
1 Use Kit
Marque
Thermo Scientific
Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).
SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the
in vivo
metabolic incorporation of heavy
Points forts
Highlights
Efficient – 100% label incorporation into proteins of living cells
Reproducible – eliminates intra-experimental variability caused by differential sample preparation
Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others
Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry
High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation
Recommended for
Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-129298
Réf. fabricant
11688833
Formule
Multi-component kit with heavy and light L-Lysine and DMEM:F12 Media
Contenus des kits
L-Lysine-2HCl, 50mg, Dialyzed FBS, 2 x 50mL, DMEM:F12 Media for SILAC, 2 x 500mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6, 50mg
Suffisant pour
One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
Quantité
1 Use Kit
À utiliser avec (application)
SILAC experiments (stable isotope labeling with amino acids in cell culture)
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