Analysez l’expression des protéines par spectrométrie de masse à l’aide de ces milieux ou additifs pour un marquage isotopique stable avec des acides aminés en culture cellulaire (SILAC).
Ces milieux de culture cellulaire et composants sériques Thermo Scientific Pierce sont optimisés pour une utilisation avec un marquage isotopique stable avec des acides aminés en culture cellulaire (SILAC) pour analyser l’expression des protéines par spectrométrie de masse (MS).
SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.Highlights:
Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat
Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry
High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation
