This kit is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples.
Quantité
100 Assays
Type de produit
Assay Kit (Colorimetric)
Contenu et stockage
Store at -20°C in the dark. Avoid freeze-thaw cycles.
Marque
Novus Biologicals
The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 μg collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method.
Kit Contents:
Caractéristiques principales
Chloramine T Concentrate
Collagen I Standard (3 mg/mL)
Microplate Sealing Film
Developer Solution
DMAB Concentrate (in DMSO)
Oxidation Buffer
Caractéristiques techniques
Marque
Novus Biologicals
Réf. B-LABO
BL-RE-130456
Réf. fabricant
18055464
Quantité
100 Assays
Type de produit
Assay Kit (Colorimetric)
Contenu et stockage
Store at -20°C in the dark. Avoid freeze-thaw cycles.
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