These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
The Hydrogen Sulfide (H2S) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of H2S in serum, plasma, and tissue samples. H2S is a crucial signal molecule involved in various biological processes, from cellular signal transduction to nerve conduction and protein synthesis, where it plays a role in regulating development, angiogenesis, and protecting organs from ischemia or reperfusion injury. In this assay H2S reacts with an acetate solution to create soluble ZnS, where, in the presence of Fe3+, methylene blue is formed (which has a maximum absorption peak at 665 nm). The concentration of iron can be indirectly calculated by measuring the OD value. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-143628 |
| Réf. fabricant | 17942141 |
| Plage de dosage | 4.86–100 μmol/L |
| Sensibilité du dosage | 2.75 μmol/L |
| Contenu et stockage | Store at 2–8°C.<br><br> |
| Méthode de détection | Colorimetric |
| Type d’échantillon | Plasma, Serum, Tissue Homogenates |
| À utiliser avec (équipement) | Microplate Reader |
| Temps d’incubation | 20 min. |
| Coefficient de variation inter-dosage | 9.9 |
| Coefficient de variation intra-dosage | 3.1 |
| Volume d’échantillon | 100 μL |
| Conditions d’expédition | Dry Ice, 2–8°C |
| Kit cible nommé | Hydrogen Sulfide (H2S) |
| Durée totale du dosage | 80 min. |
