Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e. g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e. g. ABL, FAK, JAK, and SRC families).
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-143743 |
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| Réf. fabricant | 18557824 |
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| Numéro d’adhésion | AI854630; ARCC2; AW125296; Brain-specific kinase; BSK; CEK7; CEK-7; CTPA; CTPP1; ECK; eck-like sequence 1; Ehk1; EHK-1; EK7; Ekh1; Els1; Els1.; epa5; EPH homology kinase 1; Eph receptor A5; EPHA5; EPH-like kinase 7; Ephrin A2; Ephrin A5; ephrin type-A receptor 5; EphrinA2; EphrinA5; HEK7; receptor protein-tyrosine kinase HEK7; Rek7; TYRO4; tyrosine-protein kinase receptor EHK-1 |
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| Plage de dosage | 0.33 to 80 ng/mL |
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| Sensibilité du dosage | 0.33 ng/mL |
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| Conjugué | Biotin |
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| Type de produit | ELISA |
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| Type d’échantillon | Serum |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Identification génétique (Entrez) | P54757 |
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| Symbole de gène(s) | EPHA5 |
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| Coefficient de variation inter-dosage | <12% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
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| Marqueur ou colorant | HRP |
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| Quantité | 10 x 96 Tests |
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| État réglementaire | Research Use Only |
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| Volume d’échantillon | Plasma, 50 µL; Supernatant, 50 µL; Serum, 50 µL |
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| Conditions de stockage | 2°C to 8°C |
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| Cible | EphA5 |
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| Heure du test | 1 hr 20 min |
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| Durée totale du dosage | 4 hr 45 min |
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