ErbB 4 (Her-4, c-erbB-4, v-erbB-4) is a member of the class I receptor kinase family. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-143824 |
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| Réf. fabricant | 18517794 |
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| Numéro d’adhésion | 4 ICD; ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; erb-b2 receptor tyrosine kinase 4; ERBB4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; HER4; human epidermal growth factor receptor 4; Mer4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; s80HER4; Tyro-2; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v-erb-b2 avian erythroblastic leukemia viral oncogene 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 |
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| Plage de dosage | 0.2 to 50 ng/mL |
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| Sensibilité du dosage | 0.2 ng/mL |
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| Conjugué | Biotin |
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| Type de produit | ELISA |
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| Type d’échantillon | Plasma |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Identification génétique (Entrez) | Q15303 |
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| Symbole de gène(s) | ERBB4 |
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| Coefficient de variation inter-dosage | <12% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
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| Marqueur ou colorant | HRP |
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| Quantité | 10 x 96 Tests |
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| État réglementaire | Research Use Only |
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| Volume d’échantillon | Supernatant, 50 µL; Plasma, 50 µL; Serum, 50 µL |
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| Conditions de stockage | 2°C to 8°C |
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| Cible | ErbB4 |
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| Heure du test | 1 hr 20 min |
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| Durée totale du dosage | 4 hr 45 min |
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