Sandwich ELISA Kit
The Human Chorionic Gonadotropin (hCG) ELISA quantitates Hu CG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CG. Principle of the method The Human CG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-143857 |
| Réf. fabricant | 18537814 |
| Numéro d’adhésion | P01215 |
| Plage de dosage | 54.87 to 40,000 pg/mL |
| Sensibilité du dosage | 50 pg/mL |
| Plage d’étalonnage | 50 pg/mL to 40000 pg/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Other Proteins |
| Type d’échantillon | Plasma |
| Spécificité | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Suffisant pour | 96 assays |
| Espèces cibles | Human |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 1081 |
| Symbole de gène(s) | CG-ALPHA,FSHA,GPHA1,GPHa,HCG,LHA,TSHA |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Stop Solution |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Apoptosis |
| Volume d’échantillon | Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA |
| Cible | Human |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
