Mouse lung tissue lysate, Non-denatured; Abnova

Réf. : BL-RE-147119Réf. fabricant : 16009585 200 μg

109,00 € HT

À utiliser avec (application): Immunoprecipitation, Western Blot
Tissue: Lung
Description: Whole tissue lysate (non-denatured)
Tampon de lyse: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Méthode de préparation: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Marque: Abnova

Caractéristiques techniques

Marque	Abnova
Réf. B-LABO	BL-RE-147119
Réf. fabricant	16009585
À utiliser avec (application)	Immunoprecipitation, Western Blot
Tissue	Lung
Tampon de lyse	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Méthode de préparation	Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Test du contrôle qualité	12.5% SDS-PAGE Stained with Coomassie Blue.
Quantité	200 μg
Tampon de stockage	In modified RIPA Lysis Buffer.
Espèces hôtes	Mouse
Contenu et stockage	Store at -80°C. Aliquot to avoid repeated freezing and thawing.

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Caractéristiques techniques