PtK2 (human kidney) Whole cell lysate, Non-denatured; Abnova
109,00 € HT
- À utiliser avec (application)
- Western Blot
- Tissue
- Marsupial Kidney, Normal
- Tampon de lyse
- Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF
- Méthode de préparation
- Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay
- Test du contrôle qualité
- 12.5% SDS-PAGE stained with Coomassie Blue
- Marque
- Abnova
