Hoechst Janelia Fluor™ 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor™ 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor™ 526 exhibits a quantum yield = 0.04. Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor™ 526 is suitable for multicolor microscopy experiments and for use in live cell imaging. Excitation maximum = 531 nm; emission maximum = 549 nm.
To measure the absorbance spectrum of Hoechst Janelia Fluor™ 526 we recommend the following solvent: TFE plus 0.1% TFA.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-151152 |
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| Réf. fabricant | 18716886 |
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| Nom chimique ou matériau | 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3 H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1 H,3 'H-[2,5 '-bibenzo[d]imidazol]-2 '-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)be |
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| Quantité | 100 μg |
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| Cible | Fluorescent DNA Stains |
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| Formule moléculaire | C<sub>62</sub>H<sub>58</sub>F<sub>6</sub>N<sub>10</sub>O<sub>8</sub>0.4 CF<sub>3</sub>CO<sub>2</sub>H |
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| Pureté | 0.95 |
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