Janelia Fluor® 646, free acid is a red fluorogenic fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm
et al
- see references below) for the synthesis of Janelia Fluor™ HaloTag™ and SNAP-Tag™ ligands, for use in live cell imaging experiments. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm; Quantum yield = 0.54; Extinction coefficient = 152,000 M-1cm-1.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
Caractéristiques techniques
| Marque | Tocris |
|---|
| Réf. B-LABO | BL-RE-151210 |
|---|
| Réf. fabricant | 18798746 |
|---|
| Nom chimique ou matériau | 1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9 H)-ylidene]azetidinium inner salt trifluoroacetate |
|---|
| Quantité | 5 mg |
|---|
| Cible | Free Acid Janelia Fluor™ Dyes |
|---|
| Formule moléculaire | C<sub>29</sub>H<sub>28</sub>N<sub>2</sub>O<sub>4</sub>Si.CF<sub>3</sub>CO<sub>2</sub>H |
|---|
| Poids de la formule | 3737 |
|---|
| Pureté | 95% |
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