BODIPY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BODIPY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BODIPY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BODIPY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and theTR-FRET binding assay is sensitive, selective and resistant to assay interference. BODIPY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ∼ 504 nm; emission maximum ∼ 520 nm.
BODIPY® is a registered trademark of Molecular Probes.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-151346 |
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| Réf. fabricant | 18728766 |
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| Nom chimique ou matériau | (2 S,4 R)-1-(-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5 H-5λ4,6λ4-dipyrrolo[1,2-c:2 ',1 '-f][1,3,2 ]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-ca |
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| Quantité | 2 mg |
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| Cible | Fluorescent Probes |
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| Formule moléculaire | C<sub>46</sub>H<sub>62</sub>BF<sub>2</sub>N<sub>7</sub>O<sub>9</sub>S |
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| Pureté | 95% |
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