Hoechst Janelia Fluor™ 646 is a fluorogenic red-emitting DNA probe; it preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor™ 526 for multiplexing experiments. Hoechst Janelia Fluor™ 646 can also be combined with Hoechst Janelia Fluor™ 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor™ 646 in
E. coli
cells (∽100 nM - 1 μM).
Hoechst Janelia Fluor™ 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Hoechst Janelia Fluor™ 646 exhibits a quantum yield (QY) of 0.54; extinction coefficicent 152, 000; excitation maximum = 655 nm; emission maximum = 670 nm.
Caractéristiques techniques
| Marque | Tocris Bioscience |
|---|
| Réf. B-LABO | BL-RE-151545 |
|---|
| Réf. fabricant | 18707196 |
|---|
| Nom chimique ou matériau | 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1 H,3 'H-[2,5 '-bibenzo[d]imidazol]-2 '-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoat |
|---|
| CAS | 2462011-60-3 |
|---|
| Quantité | 100 μg |
|---|
| Cible | Fluorescent DNA Stains |
|---|
| Formule moléculaire | C<sub>64</sub>H<sub>70</sub>N<sub>10</sub>O<sub>7</sub>Si |
|---|
| Pureté | 0.90 |
|---|
Une question, une configuration particulière ?
Contactez-nous pour un devis.
