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Thermo Scientific™ CaptureSelect™ tPA Affinity Matrix

Réf. : BL-RE-151872Réf. fabricant : 18476658 10 mL
1 826,00 € HT

CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA).

Capacité de liaison
8 to 10 g/L
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Granulométrie
65 ± 10 μm
Marque
Thermo Scientific

Features of this affinity matrix include:

Points forts

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect tPA affinity ligand
  • Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
  • Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
  • Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0

Caractéristiques principales

  • Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
  • Caustic cleaning with 50 mM NaOH
  • Excellent scalability
  • Non-animal-derived

Caractéristiques techniques

MarqueThermo Scientific
Réf. B-LABOBL-RE-151872
Réf. fabricant18476658
Capacité de liaison8 to 10 g/L
Débit50 to 200 cm/hr. (Up to 2 bar)
MatriceAgarose-based, epoxide-activated
Contenu et stockageStore in refrigerator (2°C to 8°C).
Granulométrie65 ± 10 μm
FormeAgarose Bead
Gamme de produitsCaptureSelect™
Quantité10 mL
TypeAffinity Matrix
Type de ligandTPA affinity ligand
Phase stationnaireAnti-tPA
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