CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect tPA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0
Caractéristiques principales
Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
Caustic cleaning with 50 mM NaOH
Excellent scalability
Non-animal-derived
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-151873
Réf. fabricant
18426608
Capacité de liaison
8 to 10 g/L
Débit
50 to 200 cm/hr. (Up to 2 bar)
Matrice
Agarose-based, epoxide-activated
Contenu et stockage
Store in refrigerator (2°C to 8°C).
Granulométrie
65 ± 10 μm
Forme
Agarose Bead
Gamme de produits
CaptureSelect™
Quantité
50 mL
Type
Affinity Matrix
Type de ligand
TPA affinity ligand
Phase stationnaire
Anti-tPA
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