This multiplexed assay enables predicted simultaneous screening of over 200 species of Mycoplasma target, discriminatory detection of an assay specific control template, and internal PCR control with no cross-reactivity to closely-related bacterial species.Features of the MycoSEQ Plus Mycoplasma Detection Kit• Provides results in less than 1 day• Includes a unique discriminatory positive control to differentiate a true positive result from a potential cross-contamination positive call• Includes protocols for various methods • Live mycoplasma is not needed: MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require use of live mycoplasma for validation• Can be used with gDNA, inactivated mycoplasma or live mycoplasma• May be used as an alternative to the standard 28-day culture test• May be used in conjunction with culture-based methods by providing preliminary results while waiting for results from the 28-day testPrepSEQ nucleic acid extraction kits offer a robust and easy workflow to overcome complex matrix challenges to ensure the required sensitivity. Use MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. A57925) for manual extractions, or MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ Express Nucleic Acid Extraction Kit (Cat. No. A57926) for automated extraction using the Automate Express Nucleic Acid Extraction System.Also available but not included with the detection kit: MycoSEQ gDNA kits (Cat. No. A49973 and A49976), containing purified genomic DNA from various mycoplasma species, may be used as positive test material during method development.Integrated workflow solutionThe MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious agent, impurity and contaminant testing during biopharmaceutical manufacturing. The entire workflow including PrepSEQ Sample Preparation Kits, which offer manual or automated sample preparation approaches, together with qPCR analysis can provide results in under 5 hours. (Please note complex matrices may require additional upfront processing steps, as described in various available protocols.)MycoSEQ Plus kit performance has been tested and confirmed on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis using AccuSEQ Real-Time PCR Detection Software provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.
Caractéristiques techniques
| Marque | Applied Biosystems |
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| Réf. B-LABO | BL-EQ-154214 |
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| Réf. fabricant | 18755455 |
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| Contenu et stockage | <b>MycoSEQ Mycoplasma Detection Kit</b><br> • 2X qPCR Master Mix Plus, -15 to -25°C<br> • MycoSEQ Plus 10X Assay Mix, -15 to -25°C<br> • Negative Control, -15 to -25°C until first thawed, then store at 2°C to 8°C<br> • DNA Dilution Buffer, -15 to -25°C until first thawed, then store at 2°C to 8°C<br> • MycoSEQ Plus Discriminatory Positive Control, -15 to -25°C<br><br> <b>PrepSEQ Express Nucleic Acid Sample Extraction Kit</b><br> • PrepSEQ Express Cartidges, store at 18-25°C<br> • Lysis Tubes, store at 18-25°C<br> • Sample Tubes, store at 18-25°C<br> • Elution Buffer, store at 18-25°C<br> • Elution Tubes, store at 18-25°C<br> • AutoMate Exptress Tips and Tip Holders, store at 18-25°C |
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| Méthode de détection | Primer-probe |
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| Forme | Liquid |
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| GC-Rich PCR Performance | Medium |
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| Méthode PCR | Multiplex qPCR |
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| Vitesse de réaction | Standard |
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| À utiliser avec (équipement) | QS5 0.1 mL Block or 7500 FAST |
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| Marqueur ou colorant | FAM |
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| Gamme de produits | MycoSEQ™ |
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| Type de produit | Mycoplasma Detection |
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| Quantité | 100 Reactions |
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| Conditions d’expédition | Dry Ice |
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| Classe d’organisme cible | Mollicutes, mycoplasma |
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| À utiliser avec (application) | Microbial Detection |
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| Nbre de réactions | 100 Reactions |
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| Type d’échantillon | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
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