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Thermo Scientific™ Pfu DNA Polymerase

Réf. : 10180690

71.50€ - 317.00€€

Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.

Description: Pfu DNA Polymerase
Quantité: 500U
À utiliser avec (application): High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
Thermostabilité: remains 95% active after 2 hours incubation at 95°C
Comprend: 2 x 1.25mL 10X Pfu Buffer with (NH4)2SO2, 2 x 1.25mL 10X Pfu Buffer with MgSO4 and 2 x 1.25mL 25mM MgSO4
Marque: Thermo Scientific™

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.

Highlights

Eight times more accurate than Taq DNA polymerase
Highly thermostable – remains 95% active after 2 hours incubation at 95°C
Generates blunt-end PCR products
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Increased PCR yields with native Pfu DNA Polymerase

Description: Pfu DNA Polymerase
Quantité: 500U
À utiliser avec (application): High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
Thermostabilité: remains 95% active after 2 hours incubation at 95°C
Comprend: 2 x 1.25mL 10X Pfu Buffer with (NH4)2SO2, 2 x 1.25mL 10X Pfu Buffer with MgSO4 and 2 x 1.25mL 25mM MgSO4

Thermo Scientific™ Pfu DNA Polymerase

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