The Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of TBARS in serum, plasma, animal tissue, cultured cells, and other sample types. TBARS are products of lipid peroxidation, a process that occurs when polyunsaturated fatty acids in cellular membranes are attacked by oxygen free radicals. TBARS, particularly malondialdehyde (MDA), are commonly used as biomarkers of lipid peroxidation and can reflect the degree of oxidative stress and cellular injury in organisms. In this assay, TBARS and thiobarbituric acid (TBA) react under high temperature and acidic conditions. This reaction forms a pink compound, which has an optical density measured at 530–540 nm and the concentration of which is linearly related to the concentration of TBARS in the sample. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-155174 |
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| Réf. fabricant | 17972061 |
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| Plage de dosage | 2.6–100 μmol/L |
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| Sensibilité du dosage | 0.85 μmol/L |
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| Contenu et stockage | Store at 2–8°C.<br><br> |
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| Méthode de détection | Colorimetric |
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| Type d’échantillon | Plasma, Serum, Tissue Homogenates |
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| À utiliser avec (équipement) | Microplate Reader |
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| Temps d’incubation | 60 min. |
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| Coefficient de variation inter-dosage | 7.1 |
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| Coefficient de variation intra-dosage | 4.3 |
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| Volume d’échantillon | 100 μL |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Thiobarbituric Acid Reactants (TBARS) |
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| Durée totale du dosage | 80 min. |
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