Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Assay IL-1a (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-1 alpha ELISA Kits.
These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA
No significant cross-reaction with human IFNa, IFNg, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, or mouse IL-1a. Human IL-1ra does not interfere with this ELISA
Suffisant pour
2 x 96 microplate wells
Contenus des kits
Streptavidin-HRP Concentrate, 2 x 75µL, TMB Substrate Solution, 2 x 13mL, Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 2 ea, 30X Wash Buffer, 2 x 50mL, Adhesive Plate Covers, 12 ea, Biotinylated Antibody Reagent, 2 x 8mL, HRP Dilution Buffer, 2 x 14mL, Lyophilized Recombinant Human IL-1 alpha Standard, 4 vials, Standard Diluent, 2 x 12mL, Stop Solution, 2 x 13mL
Mesure
Absorbance at 450nm minus absorbance at 550nm
Précision
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Quantité
2 Plate Kit
Volume d’échantillon
50 μL
Cible
Natural and recombinant human interleukin-1 alpha (IL-1a)
Durée totale du dosage
3 hr.
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